Product Overview
The ChromaFlash Chromatin Extraction Package deal is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle could be utilized in various chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Package deal. The isolated chromatin may also be utilized in several chromatin-related features equal to in vitro protein-DNA binding assays and nuclear enzyme assays.
- Terribly fast course of: your complete course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
- Helpful and versatile: the bundle is acceptable for preparing every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
- Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.

Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, up to date and frozen tissues, and so forth. The amount of cells and tissues for each preparation might be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues. Yield of chromatin is roughly 4 ug per 106 cells or per 50 mg tissues.
Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous gadget for locating out protein-DNA interaction. With ChIP, the experimenter can resolve if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, a ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly in demand.
Principle & Course of
The ChromaFlash™ Plant Chromatin Extraction Package deal accommodates the entire reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.
miRNA Extraction Kit |
K1456-50 | Biovision | 50 Preps | EUR 661.2 |
gPURE EXTRACTION KIT |
IB47430 | IBI Scientific | 3ML | EUR 30.42 |
Description: (FOR CULTURED CELLS) |
gPURE EXTRACTION KIT |
IB47431 | IBI Scientific | 100ML | EUR 196.06 |
Description: (FOR CULTURED CELLS) |
gPURE EXTRACTION KIT |
IB47432 | IBI Scientific | 1000ML | EUR 1358.49 |
Description: (FOR CULTURED CELLS) |
ExoDNA? Extraction Kit |
K1242-25 | Biovision | each | EUR 705.6 |
ExoDNA? Extraction Kit |
K1242-50 | Biovision | each | EUR 1057.2 |
Chromatin protein Cren7 Antibody (HRP) |
20-abx109204 | Abbexa | - EUR 493.20
- EUR 2214.00
- EUR 718.80
- EUR 218.40
- EUR 360.00
| - 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
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Chromatin protein Cren7 Antibody (FITC) |
20-abx107787 | Abbexa | - EUR 493.20
- EUR 2214.00
- EUR 718.80
- EUR 218.40
- EUR 360.00
| - 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Up RNA Extraction Kit |
20-abx098087 | Abbexa | | |
|
Chromatin Modifying Protein 2A Protein |
20-abx261989 | Abbexa | - EUR 5388.00
- EUR 393.60
- EUR 276.00
| |
|
Chromatin Modifying Protein 2B Protein |
20-abx262025 | Abbexa | - EUR 5388.00
- EUR 393.60
- EUR 276.00
| |
|
Chromatin Modifying Protein 4A Protein |
20-abx263203 | Abbexa | - EUR 276.00
- EUR 1779.60
- EUR 393.60
| |
|
Chromatin Modifying Protein 1A Protein |
20-abx263326 | Abbexa | - EUR 1930.80
- EUR 393.60
- EUR 276.00
| |
|
Chromatin Protein Cren7 (creN7) Antibody |
20-abx109437 | Abbexa | - EUR 493.20
- EUR 2214.00
- EUR 718.80
- EUR 218.40
- EUR 360.00
| - 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
ATRX, Chromatin Remodeler (ATRX) Antibody |
20-abx325123 | Abbexa | | |
|
ATRX, Chromatin Remodeler (ATRX) Antibody |
abx331300-100ul | Abbexa | 100 ul | EUR 510 |
|
Chromatin protein Cren7 Antibody (Biotin) |
20-abx106375 | Abbexa | - EUR 493.20
- EUR 2214.00
- EUR 718.80
- EUR 218.40
- EUR 360.00
| - 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Quick Gel Extraction Kit |
20-abx098085 | Abbexa | | |
|
Plant RNA extraction Kit |
20-abx098088 | Abbexa | | |
|
Gel Extraction Kit (100 preps) |
9K-006-0001 | Bio Basic | 100preps | EUR 283.42 |
|
Gel Extraction Kit (200 preps) |
9K-006-0002 | Bio Basic | 200preps, 200prep | EUR 418.09 |
|
EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit |
P-2003 | EpiGentek | - EUR 1084.86
- EUR 312.40
- EUR 500.50
- EUR 738.10
| - 96 Reactions
- 24 Reactions
- 48 Reactions
- 96 Reactions
|
Chromatin Accessibility Complex 1 Protein |
20-abx261034 | Abbexa | - EUR 4101.60
- EUR 393.60
- EUR 276.00
| |
|
FFPE Protein Extraction Kit |
BSP058 | Bio Basic | 50Preps | EUR 122.64 |
|
Exosomal RNA Extraction Kit |
P200R,-part-of-P200 | 101Bio | - | Ask for price |
Exosomal DNA Extraction Kit |
P230-25 | 101Bio | - | Ask for price |