MethylFlash Methylated DNA Quantification Equipment (Colorimetric)

methylflash methylated dna quantification kit

Product Choices
The MethylFlash Methylated DNA Quantification Gear (Colorimetric) is an extra refinement of our earlier Methylamp worldwide DNA methylation quantification kits by simplifying the workflow and enhancing the consistency of outcomes. It makes use of an revolutionary methodology to permit background indicators to be terribly low, eliminating the plate drying and blocking steps. Compared with chromatography-based methods similar to HPLC or mass spectrometry, this product provides an inexpensive technique to exactly measure ranges of 5-methylcytosine. The package deal has the subsequent advantages and choices:

  • Colorimetric assay with easy-to-follow steps for consolation and velocity. The entire course of is likely to be accomplished inside 4 hours.
  • Revolutionary package deal composition permits background indicators to be terribly low, which eliminates the need for plate blocking and permits the assay to be simple, appropriate, reliable, and fixed.
  • Extreme sensitivity, of which the detection prohibit is likely to be as little as 0.2 ng of methylated DNA and accepts as little as 50 ng of enter genomic DNA.
  • Optimized antibody and enhancer choices allow extreme specificity to 5mC, with no cross-reactivity to unmethylated cytosine and no or negligible cross-reactivity to hydroxymethylcytosine all through the indicated focus fluctuate of the sample DNA.
  • Widespread optimistic and hostile controls are included, which might be acceptable for quantifying methylated DNA from any species.
  • Strip-well microplate format makes the assay versatile: handbook or extreme throughput analysis.

The MethylFlash Methylated DNA Quantification Gear (Colorimetric) is a whole set of optimized buffers and reagents to colorimetrically quantify worldwide DNA methylation by significantly measuring ranges of 5-methylcytosine (5-mC) in an ELISA-like microplate-based format. Compared with LUMA or LINE-1, Alu, and LTR-based assays, MethylFlash know-how immediately quantifies exact worldwide DNA methylation.

This package deal could be significantly optimized for paired use with the MethylFlash Hydroxymethylated DNA Quantification Gear (Colorimetric) for concurrently quantifying every methylated DNA and hydroxymethylated DNA.

 

Principle & Course of
The MethylFlash Methylated DNA Quantification Gear (Colorimetric) includes all reagents essential for the quantification of world DNA methylation. On this assay, DNA is certain to strip wells which could be significantly dealt with to have a extreme DNA affinity.

The methylated fraction of DNA is detected using seize and detection antibodies after which quantified by an ELISA-like response by finding out the absorbance in a microplate spectrophotometer at 450 nm. The amount of methylated DNA is proportional to the OD depth measured, which is likely to be calculated with the package deal’s included formulation for relative methylation standing of two completely totally different DNA samples or absolute quantification of 5-methylcytosine (5mC) using an extraordinary curve.

methylflash methylated dna quantification kit

methylflash methylated dna quantification package

Easy, Fast, and Versatile
The entire colorimetric assay has easy-to-follow steps for consolation and velocity, which is likely to be completed in 4 hours. The strip-well microplate format permits for a flexible assay in handbook or extreme throughput analysis. Widespread optimistic and hostile controls are included with the package deal for quantifying methylated DNA from any species similar to mammals, vegetation, fungi, micro organism, and viruses.

Safe and Useful
All the wished reagents, along with hostile controls and optimistic controls, for quantification of world DNA methylation are conveniently packaged throughout the package deal. The direct colorimetric quantification of DNA samples replaces outdated or inferior methods and eliminates the need for DNA digestion/denaturation, radioactivity, extraction, or chromatography.

 

 

Extraordinarily Delicate and Specific
The novel course of and proprietary package deal compositions allow for proper quantification of methylated DNA to be achieved with extreme sensitivity and specificity. The detection prohibit of the enter DNA is likely to be as little as 0.2 ng of methylated DNA.

Comparability of On the market ELISA-based World DNA Methylation Assay Methods

MethylFlash(Colorimetric) Competitor Z Competitor C
Assay Principle Direct ELISA Direct ELISA Indirect ELISA
Format 96-well plate 96-well plate 96-well plate
Sensitivity Superb detection prohibit: 0.2 ng of 5-mC DNA Good detection prohibit: >0.5 ng of 5-mC DNA Very poor detection prohibit: >1000 ng of 5-mC DNA
S/N Ratio >20 with very low background  <4 with extreme background  N/A*
Procedural Consolation No need for plate blocking and denaturation of DNA Requires plate blocking and DNA denaturation Requires plate blocking, DNA digestion, and antibody aggressive incubation in an additional plate
Protocol Time  <4 hr  <4 hr  >eight hr
DNA Kind & Species Widespread for any species, every ssDNA and dsDNA Human and mouse ssDNA solely Quite a few species
Specificity Specific to 5-mC solely Cross-reaction to 5-hmC N/A*
Quantitation Kind Every relative and absolute quantification Relative Relative
Commonplace Administration Safe, quantitative in absolute amount of 5-mC, and is likely to be universally used for any species Unstable, cannot be quantitative in amount, and will solely be used for human or mouse N/A*
Minimal Enter Amount 50 ng 100 ng 1000 ng
Accuracy of Detection Extreme, regular and quantified commonplace administration. Confirmed shut correlation with LC-MS analysis by clients Unclear, unstable and un-quantified commonplace administration N/A*
% 5-mC calculation Simple Subtle Not Supplied
Patented Methodology Certain No No
Recognition Very extreme revealed citation rely Low revealed citation rely Low revealed citation rely
Assist Expertise Since 2006 Since 2013 Since 2011

 

DNA Methylation Quantification

DNA methylation performs a vital place in common organismal enchancment and in cellular differentiation in higher organisms. Gene expression, along with the occasion of virtually every kind of most cancers, are moreover tied to DNA methylation.

As an illustration, worldwide decrease in 5-methylcytosine content material materials (DNA hypomethylation) might be going attributable to methyl-deficiency as a consequence of numerous environmental influences and has been proposed as a molecular marker in a lot of natural processes similar to most cancers. World quantification of DNA methylation is crucial for understanding the roles that gene expression and silencing play throughout the enchancment of most cancers and totally different illnesses.

 

Individual Reaction Mix 9

G065-9 200 reactions
EUR 200.4

Mouse Brain Tissue Preparation Buffer 9: Normal Nerve Microglia

9-80132 1 x 100 ml Ask for price

7095D 9 PASTEUR PIPET BOROSILICATE

7095D-9 200/pk
EUR 93.6
Description: Disposable Pipets; Pasteur Pipets

Porcine CCL-5 ELISA Kit

EPC0240 96Tests
EUR 625.2

Rabbit CCL-5 ELISA Kit

ERTC0240 96Tests
EUR 625.2

Rat CCL-5 ELISA Kit

ERC0240 96Tests
EUR 625.2

Monkey CCL-5 ELISA Kit

EMKC0240 96Tests
EUR 625.2

Human CCL-5 ELISA Kit

EHC0240 96Tests
EUR 625.2

Goat CCL-5 ELISA Kit

EGTC0240 96Tests
EUR 625.2

Canine CCL-5 ELISA Kit

ECC0240 96Tests
EUR 625.2

Chicken CCL-5 ELISA Kit

ECKC0240 96Tests
EUR 625.2

Anserini CCL-5 ELISA Kit

EAC0240 96Tests
EUR 625.2

Bovine CCL-5 ELISA Kit

EBC0240 96Tests
EUR 625.2

Sheep CCL-5 ELISA Kit

ESC0240 96Tests
EUR 625.2

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 336

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 336

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 336

Guinea Pig CCL-5 ELISA Kit

EGC0240 96Tests
EUR 625.2

Human Brain Tissue Preparation Buffer 9: Normal Nerve Microglia

9-80021 1 x 100 ml Ask for price

Human Cancer Tissue Preparation Buffer 9: Kidney Tumor Cells

9-80032 1 x 100 ml Ask for price

Rat Brain Tissue Preparation Buffer 9: Normal Neuron Cells

9-80227 1 x 100 ml Ask for price

Nori® Mouse CCL-15 (MIP-5) ELISA Kit

GR117261 96-well
EUR 553.2

CF568 goat anti-mouse IgG(H+L)

9-20100
  • EUR 271.20
  • EUR 277.20
  • 0.5mL
  • 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 goat anti-mouse IgG(H+L)

9-20110
  • EUR 271.20
  • EUR 277.20
  • 0.5mL
  • 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 goat anti-mouse IgG(H+L)

9-20120
  • EUR 271.20
  • EUR 277.20
  • 0.5mL
  • 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF350 goat anti-mouse IgG(H+L)

9-20140
  • EUR 271.20
  • EUR 277.20
  • 0.5mL
  • 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF405M goat anti-mouse IgG(H+L)

9-20180
  • EUR 271.20
  • EUR 277.20
  • 0.5 mL
  • 1mg
Description: Minimum order quantity: 1 unit of 1mg

Biotin goat anti-mouse IgG(H+L)

9-20183
  • EUR 271.20
  • EUR 277.20
  • 0.5mL
  • 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF640R goat anti-mouse IgG(H+L)

9-20197
  • EUR 271.20
  • EUR 277.20
  • 0.5mL
  • 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF488A chicken anti-mouse IgG(H+L)

9-20208
  • EUR 271.20
  • EUR 277.20
  • 0.5mL
  • 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF594 chicken anti-mouse IgG(H+L)

9-20221
  • EUR 271.20
  • EUR 277.20
  • 0.5mL
  • 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF633 chicken anti-mouse IgG(H+L)

9-20222
  • EUR 271.20
  • EUR 277.20
  • 0.5mL
  • 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF543 goat anti-mouse IgG(H+L)

9-20306
  • EUR 271.20
  • EUR 277.20
  • 0.5mL
  • 1mg
Description: Minimum order quantity: 1 unit of 1mg

CF488A goat anti-mouse IgG (H+L)

9-20010
  • EUR 271.20
  • EUR 277.20
  • 0.5 mL
  • 1mg
Description: Minimum order quantity: 1 unit of 1mg

 

 

Related Post

transgenicnews

Replacing the consumption of red meat with other major dietary protein sources and risk of type 2 diabetes mellitus: a prospective cohort studyReplacing the consumption of red meat with other major dietary protein sources and risk of type 2 diabetes mellitus: a prospective cohort study

Background: Better consumption of crimson meat has been related to the next danger of sort 2 diabetes mellitus (T2DM). A decreased consumption of crimson meat and simultaneous elevated consumption of different