Our Titration Kits current a fast and straightforward approach for titrating your virus. The kits make use of each a quick RNA or DNA extraction step, and resolve viral RNA/DNA titer using qRT-PCR.
Advantages of abm’s titration kits:
- No RNA purification — saves time and eliminates inaccuracy.
- Ready-to-use reagent mix — reduces variability.
- qRT-PCR in a single step — additional delicate and proper than completely different methods.
- No NTC amplification — Distinctive primer design completely eliminates non-specific amplification in NTCs.
We moreover provide On the spot Lentivirus Titration Taking part in playing cards, which allow quick detection of lentiviral capsule proteins as a marker to substantiate the presence of lentivirus. Utilizing the lateral flow into immunoassay approach, viral titer yields are assessed instantly inside 10 minutes using our three step protocol.
he Retro-X qRT-PCR Titration Tools provides a fast and straightforward approach for measuring retrovirus titer. The kits use a quick RNA purification step sooner than quantifying the number of retroviral genome copies using qRT-PCR and TB Inexperienced utilized sciences.
Description
abm’s qPCR Retrovirus Titer Tools is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and might be completed beneath one hour. Designed to ship extreme sensitivity and specificity, the tools ensures minimal non-specific background and better complete effectivity as compared with comparable kits within the market. ROX reference dye is obtainable separate from the MasterMix, making this tools universally appropriate with most qPCR gadgets.
PRODUCT COMPONENT |
QUANTITY |
BlasTaq 2X qPCR Titer MasterMix |
1.25 ml |
Primer Mix |
100 rxn (200 µl) |
Commonplace Administration DNA |
50 µl |
ROX Reference Dye |
15 µl |
Nuclease-Free H2O |
2 x 1.zero ml |
SKU |
G949 |
Unit quantity |
100 rxn |
Storage State of affairs |
Retailer at -20°C |
|

qPCR and One-Step RT MasterMixes
Quick willpower of retroviral titer
Whereas standard titration methods require as a lot as 10 days to complete, these titration kits require solely 4 hours and work with all MMLV-based retroviral vectors (not MSCV). Using qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an an infection, allowing you to hold out every on the equivalent day.
Receive fixed retroviral transductions
A critical good thing about retroviral gene-delivery strategies is the facility to manage the extent of gene expression by adjusting the multiplicity of an an infection (MOI) to dictate the number of viral genomes that enter your objective cells. These titration kits allow you to make a quick correlation between the retroviral copy amount and retroviral infectivity in your cell type and in the long run allow you to administration the expression stage between experiments.
The best way it really works
The qRT-PCR course of entails subjecting serial dilutions of your sample and a administration RNA of acknowledged copy amount (provided) to one-step qRT-PCR. The retroviral copy amount contained in your sample can then be determined by evaluating its Ct value to the standard curve.
RNA purification and DNase I remedy steps are essential to the protocol so even viruses packaged using transient transfections, which always embrace residual plasmid DNA, might be exactly quantified using this tools.
Retrovirus Mini Purification Kit |
K1307-10 |
Biovision |
each |
EUR 652.8 |
Retrovirus Mini Purification Kit |
K1307-20 |
Biovision |
each |
EUR 1096.8 |
Retrovirus Maxi Purification Kit |
K1308-10 |
Biovision |
each |
EUR 1554 |
Retrovirus Maxi Purification Kit |
K1308-2 |
Biovision |
each |
EUR 639.6 |
Retrovirus Maxi Purification Kit |
K1308-4 |
Biovision |
each |
EUR 1032 |
Retrovirus Booster 50µl |
ABP-RVC-RBST05 |
Allele Biotech |
50µl |
Ask for price |
|
Retrovirus Booster 200µl |
ABP-RVC-RBST20 |
Allele Biotech |
200µl |
Ask for price |
|
Retrovirus Booster 400µl |
ABP-RVC-RBST40 |
Allele Biotech |
400µl |
Ask for price |
|
Retrovirus Stabilizer 50µl |
ABP-RVC-RSTB05 |
Allele Biotech |
50µl |
Ask for price |
|
Retrovirus Stabilizer 200µl |
ABP-RVC-RSTB20 |
Allele Biotech |
200µl |
Ask for price |
|
Retrovirus Stabilizer 400µl |
ABP-RVC-RSTB40 |
Allele Biotech |
400µl |
Ask for price |
|
iPS Cell Line (Retrovirus) |
ASE-9101 |
Applied StemCell |
1 vial of ^2.5X10^5 cells |
EUR 516.84 |
Description: 6 month |
QuickTiter Retrovirus Quantitation Kit |
VPK-120 |
Cell Biolabs |
20 assays |
EUR 853.2 |
Description: Our QuickTiter Retrovirus Quantitation Kit provides a quick method for determining retroviral titer. This assay measures the viral nucleic acid content of your retrovirus, and may be performed either before or after purification of your virus. |
M13 Phage Titration ELISA Kit |
55R-PRPHAGE |
Fitzgerald |
96 tests |
EUR 831.6 |
Description: Enzyme Immunoassay for the Quantitative Determination of purified M13 Bacteriophage Particles |
Lentivirus and Retrovirus Purification Kit |
K1458-100 |
Biovision |
100 Preps |
EUR 1244.4 |
Lentivirus and Retrovirus Purification Kit |
K1458-20 |
Biovision |
20 Preps |
EUR 727.2 |
Retrovirus-related Gag Antibody |
48261-100ul |
SAB |
100ul |
EUR 399.6 |
Retrovirus-related Gag Antibody |
48261-50ul |
SAB |
50ul |
EUR 286.8 |
Rat Serum IgG detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
80155-G |
Alpha Diagnostics |
1 kit |
EUR 781.2 |
Rat Serum IgM detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
80155-M |
Alpha Diagnostics |
1 kit |
EUR 781.2 |
Mouse Serum IgM detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
80150-M |
Alpha Diagnostics |
1 kit |
EUR 781.2 |
Mouse Serum IgG3 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
80150-G3 |
Alpha Diagnostics |
1 kit |
EUR 781.2 |
Retrovirus Concentration Solution (100 mL) |
P905C |
101Bio |
- |
Ask for price |
Endogenous retrovirus (replication competent) PCR kit |
PCR-V725-48D |
Bioingentech |
50T |
EUR 510.96 |
|
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent) |
Endogenous retrovirus (replication competent) PCR kit |
PCR-V725-96D |
Bioingentech |
100T |
EUR 625.8 |
|
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent) |
Endogenous retrovirus (replication competent) PCR kit |
PCR-H725-48D |
Bioingentech |
48T |
EUR 543.6 |
Endogenous retrovirus (replication competent) PCR kit |
PCR-H725-96D |
Bioingentech |
96T |
EUR 686.4 |
pCHAC-hKLF4 Retrovirus Vector Validated |
ABP-SC-VCREKLF |
Allele Biotech |
10µg |
Ask for price |
|
pCHAC-hSOX2 Retrovirus Vector Validated |
ABP-SC-VCRESOX |
Allele Biotech |
10µg |
Ask for price |
|
pCHAC-hLIN28 Retrovirus Vector Validated |
ABP-SC-VCRELIN |
Allele Biotech |
10µg |
Ask for price |
|
pCHAC-hNANOG Retrovirus Vector Validated |
ABP-SC-VCRENAN |
Allele Biotech |
10µg |
Ask for price |
|
ViraDuctin Retrovirus Transduction Kit (200 Transductions) |
RV-201 |
Cell Biolabs |
200 transductions |
EUR 936 |
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells. |
Mouse Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
80150-A |
Alpha Diagnostics |
1 kit |
EUR 781.2 |
Mouse Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
80150-E |
Alpha Diagnostics |
1 kit |
EUR 781.2 |
Rat Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
80155-A |
Alpha Diagnostics |
1 kit |
EUR 781.2 |
Rat Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
80155-E |
Alpha Diagnostics |
1 kit |
EUR 781.2 |
Retrovirus Restriction Factor Detection Set |
PSI-1817 |
ProSci |
1 Set |
EUR 1287.6 |
|
Description: Mammalian cells have developed multiple strategies to limit retroviral infection including numerous proteins termed restriction factors that restrict retrovirus replication and infection. One such protein is TRIM5, a member of a broad family of otherwise unrelated proteins whose longest isoform, TRIM5α, enables resistance to infection by HIV-1 through rapid degradation of HIV-1 Gag polyproteins. Another protein, APOBEC3G (and to a lesser extent APOBEC3F) can be incorporated into HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity. The human uracil-DNA glycosylase UNG2 can also be incorporated into the HIV-1virion, indicating that it is required to remove uracils from the viral genome. It has been suggested that the UNG2 contributes to the APOBEC3G-mediated loss of infectivity by generating abasic sites in the viral genome. UNG1, the mitochondrial form of UNG, is transcribed from the same gene as UNG2 through differentially regulated promoters and alternative splicing, but does not appear to have anti-retroviral properties. AID, a protein related to APOBEC3 also possesses cytidine deaminase activity that can be blocked by the HIV-1 Vif protein in E. coli, but so far it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.;;For images please see PDF data sheet |
ViraDuctin Retrovirus Transduction Kit (40 Transductions) |
RV-200 |
Cell Biolabs |
40 transductions |
EUR 435.6 |
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells. |
Endogenous retrovirus (replication competent) RT PCR kit |
RTq-H725-100D |
Bioingentech |
100T |
EUR 860.4 |
Endogenous retrovirus (replication competent) RT PCR kit |
RTq-H725-150D |
Bioingentech |
150T |
EUR 969.6 |