qPCR and One-Step RT MasterMixes

Virus Titration Kits qpcr retrovirus titration kit

Our Titration Kits current a fast and straightforward approach for titrating your virus. The kits make use of each a quick RNA or DNA extraction step, and resolve viral RNA/DNA titer using qRT-PCR.

Advantages of abm’s titration kits:

  • No RNA purification — saves time and eliminates inaccuracy.
  • Ready-to-use reagent mix — reduces variability.
  • qRT-PCR in a single step — additional delicate and proper than completely different methods.
  • No NTC amplification — Distinctive primer design completely eliminates non-specific amplification in NTCs.

We moreover provide On the spot Lentivirus Titration Taking part in playing cards, which allow quick detection of lentiviral capsule proteins as a marker to substantiate the presence of lentivirus. Utilizing the lateral flow into immunoassay approach, viral titer yields are assessed instantly inside 10 minutes using our three step protocol.

he Retro-X qRT-PCR Titration Tools provides a fast and straightforward approach for measuring retrovirus titer. The kits use a quick RNA purification step sooner than quantifying the number of retroviral genome copies using qRT-PCR and TB Inexperienced utilized sciences.

Description

abm’s qPCR Retrovirus Titer Tools is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and might be completed beneath one hour. Designed to ship extreme sensitivity and specificity, the tools ensures minimal non-specific background and better complete effectivity as compared with comparable kits within the market. ROX reference dye is obtainable separate from the MasterMix, making this tools universally appropriate with most qPCR gadgets.

PRODUCT COMPONENT QUANTITY
BlasTaq 2X qPCR Titer MasterMix 1.25 ml
Primer Mix 100 rxn (200 µl)
Commonplace Administration DNA 50 µl
ROX Reference Dye 15 µl
Nuclease-Free H2O 2 x 1.zero ml

 

SKU G949
Unit quantity 100 rxn
Storage State of affairs Retailer at -20°C

 

qPCR and One-Step RT MasterMixes

qPCR and One-Step RT MasterMixes

Quick willpower of retroviral titer

Whereas standard titration methods require as a lot as 10 days to complete, these titration kits require solely 4 hours and work with all MMLV-based retroviral vectors (not MSCV). Using qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an an infection, allowing you to hold out every on the equivalent day.

Receive fixed retroviral transductions

A critical good thing about retroviral gene-delivery strategies is the facility to manage the extent of gene expression by adjusting the multiplicity of an an infection (MOI) to dictate the number of viral genomes that enter your objective cells. These titration kits allow you to make a quick correlation between the retroviral copy amount and retroviral infectivity in your cell type and in the long run allow you to administration the expression stage between experiments.

The best way it really works

The qRT-PCR course of entails subjecting serial dilutions of your sample and a administration RNA of acknowledged copy amount (provided) to one-step qRT-PCR. The retroviral copy amount contained in your sample can then be determined by evaluating its Ct value to the standard curve.

RNA purification and DNase I remedy steps are essential to the protocol so even viruses packaged using transient transfections, which always embrace residual plasmid DNA, might be exactly quantified using this tools.

 

Retrovirus Mini Purification Kit
K1307-10 each
EUR 652.8

Retrovirus Mini Purification Kit
K1307-20 each
EUR 1096.8

Retrovirus Maxi Purification Kit
K1308-10 each
EUR 1554

Retrovirus Maxi Purification Kit
K1308-2 each
EUR 639.6

Retrovirus Maxi Purification Kit
K1308-4 each
EUR 1032

Retrovirus Booster 50µl
ABP-RVC-RBST05 50µl Ask for price

Retrovirus Booster 200µl
ABP-RVC-RBST20 200µl Ask for price

Retrovirus Booster 400µl
ABP-RVC-RBST40 400µl Ask for price

Retrovirus Stabilizer 50µl
ABP-RVC-RSTB05 50µl Ask for price

Retrovirus Stabilizer 200µl
ABP-RVC-RSTB20 200µl Ask for price

Retrovirus Stabilizer 400µl
ABP-RVC-RSTB40 400µl Ask for price

iPS Cell Line (Retrovirus)
ASE-9101 1 vial of ^2.5X10^5 cells
EUR 516.84
Description: 6 month

QuickTiter Retrovirus Quantitation Kit
VPK-120 20 assays
EUR 853.2
Description: Our QuickTiter Retrovirus Quantitation Kit provides a quick method for determining retroviral titer. This assay measures the viral nucleic acid content of your retrovirus, and may be performed either before or after purification of your virus.

M13 Phage Titration ELISA Kit
55R-PRPHAGE 96 tests
EUR 831.6
Description: Enzyme Immunoassay for the Quantitative Determination of purified M13 Bacteriophage Particles

Lentivirus and Retrovirus Purification Kit
K1458-100 100 Preps
EUR 1244.4

Lentivirus and Retrovirus Purification Kit
K1458-20 20 Preps
EUR 727.2

Retrovirus-related Gag Antibody
48261-100ul 100ul
EUR 399.6

Retrovirus-related Gag Antibody
48261-50ul 50ul
EUR 286.8

Rat Serum IgG detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80155-G 1 kit
EUR 781.2

Rat Serum IgM detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80155-M 1 kit
EUR 781.2

Mouse Serum IgM detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80150-M 1 kit
EUR 781.2

Mouse Serum IgG3 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80150-G3 1 kit
EUR 781.2

Retrovirus Concentration Solution (100 mL)
P905C - Ask for price

Endogenous retrovirus (replication competent) PCR kit
PCR-V725-48D 50T
EUR 510.96
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent)

Endogenous retrovirus (replication competent) PCR kit
PCR-V725-96D 100T
EUR 625.8
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent)

Endogenous retrovirus (replication competent) PCR kit
PCR-H725-48D 48T
EUR 543.6

Endogenous retrovirus (replication competent) PCR kit
PCR-H725-96D 96T
EUR 686.4

pCHAC-hKLF4 Retrovirus Vector Validated
ABP-SC-VCREKLF 10µg Ask for price

pCHAC-hSOX2 Retrovirus Vector Validated
ABP-SC-VCRESOX 10µg Ask for price

pCHAC-hLIN28 Retrovirus Vector Validated
ABP-SC-VCRELIN 10µg Ask for price

pCHAC-hNANOG Retrovirus Vector Validated
ABP-SC-VCRENAN 10µg Ask for price

ViraDuctin Retrovirus Transduction Kit (200 Transductions)
RV-201 200 transductions
EUR 936
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.

Mouse Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80150-A 1 kit
EUR 781.2

Mouse Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80150-E 1 kit
EUR 781.2

Rat Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80155-A 1 kit
EUR 781.2

Rat Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)
80155-E 1 kit
EUR 781.2

Retrovirus Restriction Factor Detection Set
PSI-1817 1 Set
EUR 1287.6
Description: Mammalian cells have developed multiple strategies to limit retroviral infection including numerous proteins termed restriction factors that restrict retrovirus replication and infection. One such protein is TRIM5, a member of a broad family of otherwise unrelated proteins whose longest isoform, TRIM5α, enables resistance to infection by HIV-1 through rapid degradation of HIV-1 Gag polyproteins. Another protein, APOBEC3G (and to a lesser extent APOBEC3F) can be incorporated into HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity. The human uracil-DNA glycosylase UNG2 can also be incorporated into the HIV-1virion, indicating that it is required to remove uracils from the viral genome. It has been suggested that the UNG2 contributes to the APOBEC3G-mediated loss of infectivity by generating abasic sites in the viral genome. UNG1, the mitochondrial form of UNG, is transcribed from the same gene as UNG2 through differentially regulated promoters and alternative splicing, but does not appear to have anti-retroviral properties. AID, a protein related to APOBEC3 also possesses cytidine deaminase activity that can be blocked by the HIV-1 Vif protein in E. coli, but so far it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.;;For images please see PDF data sheet

ViraDuctin Retrovirus Transduction Kit (40 Transductions)
RV-200 40 transductions
EUR 435.6
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.

Endogenous retrovirus (replication competent) RT PCR kit
RTq-H725-100D 100T
EUR 860.4

Endogenous retrovirus (replication competent) RT PCR kit
RTq-H725-150D 150T
EUR 969.6