Our Titration Kits current a fast and straightforward approach for titrating your virus. The kits make use of each a quick RNA or DNA extraction step, and resolve viral RNA/DNA titer using qRT-PCR.
Advantages of abm’s titration kits:
- No RNA purification — saves time and eliminates inaccuracy.
- Ready-to-use reagent mix — reduces variability.
- qRT-PCR in a single step — additional delicate and proper than completely different methods.
- No NTC amplification — Distinctive primer design completely eliminates non-specific amplification in NTCs.
We moreover provide On the spot Lentivirus Titration Taking part in playing cards, which allow quick detection of lentiviral capsule proteins as a marker to substantiate the presence of lentivirus. Utilizing the lateral flow into immunoassay approach, viral titer yields are assessed instantly inside 10 minutes using our three step protocol.
he Retro-X qRT-PCR Titration Tools provides a fast and straightforward approach for measuring retrovirus titer. The kits use a quick RNA purification step sooner than quantifying the number of retroviral genome copies using qRT-PCR and TB Inexperienced utilized sciences.
Description
abm’s qPCR Retrovirus Titer Tools is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and might be completed beneath one hour. Designed to ship extreme sensitivity and specificity, the tools ensures minimal non-specific background and better complete effectivity as compared with comparable kits within the market. ROX reference dye is obtainable separate from the MasterMix, making this tools universally appropriate with most qPCR gadgets.
| PRODUCT COMPONENT | QUANTITY |
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| BlasTaq 2X qPCR Titer MasterMix | 1.25 ml | | Primer Mix | 100 rxn (200 µl) | | Commonplace Administration DNA | 50 µl | | ROX Reference Dye | 15 µl | | Nuclease-Free H2O | 2 x 1.zero ml |
| SKU | G949 |
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| Unit quantity | 100 rxn |
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| Storage State of affairs | Retailer at -20°C |
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qPCR and One-Step RT MasterMixes
Quick willpower of retroviral titer
Whereas standard titration methods require as a lot as 10 days to complete, these titration kits require solely 4 hours and work with all MMLV-based retroviral vectors (not MSCV). Using qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an an infection, allowing you to hold out every on the equivalent day.
Receive fixed retroviral transductions
A critical good thing about retroviral gene-delivery strategies is the facility to manage the extent of gene expression by adjusting the multiplicity of an an infection (MOI) to dictate the number of viral genomes that enter your objective cells. These titration kits allow you to make a quick correlation between the retroviral copy amount and retroviral infectivity in your cell type and in the long run allow you to administration the expression stage between experiments.
The best way it really works
The qRT-PCR course of entails subjecting serial dilutions of your sample and a administration RNA of acknowledged copy amount (provided) to one-step qRT-PCR. The retroviral copy amount contained in your sample can then be determined by evaluating its Ct value to the standard curve.
RNA purification and DNase I remedy steps are essential to the protocol so even viruses packaged using transient transfections, which always embrace residual plasmid DNA, might be exactly quantified using this tools.
 AAV3 Titration ELISA Kit |
| AAV-A003-96tests | ACROBIOSYSTEMS | 96tests | EUR 610.4 |
Description: AAV3 Titration ELISA Kit is based on ELISA sandwich method and designed to detect and quantitative determine of AAV3 capsid titration in AAV gene therapy product preparation processing. It contains AAV3 empty capsids and a pair of antibodies against AAV3. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of AAV3 capsid titration. |
 AAV5 Titration ELISA Kit |
| AAV-A005 | ACROBIOSYSTEMS | 96tests | EUR 610.4 |
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Description: AAV5 Titration ELISA Kit is based on ELISA sandwich method and designed to detect and quantitative determine of AAV5 capsid titration in AAV gene therapy product preparation processing. It contains AAV5 empty capsids and a pair of antibodies against AAV5. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of AAV5 capsid titration. |
AAV5 Titration ELISA Kit |
| AAV-A005-96tests | ACROBIOSYSTEMS | 96tests | EUR 610.4 |
Description: AAV5 Titration ELISA Kit is based on ELISA sandwich method and designed to detect and quantitative determine of AAV5 capsid titration in AAV gene therapy product preparation processing. It contains AAV5 empty capsids and a pair of antibodies against AAV5. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of AAV5 capsid titration. |
 AAV8 Titration ELISA Kit |
| DEIAAV8 | Creative Diagnostics | 96T | EUR 624 |
Description: cell culture supernatants, purified virus preparations |
 AAV9 Titration ELISA Kit |
| DEIAAV9 | Creative Diagnostics | 96T | EUR 624 |
Description: cell culture supernatants, purified virus preparations |
 M13 Phage Titration ELISA Kit |
| 55R-PRPHAGE | Fitzgerald | 96 tests | EUR 699 |
Description: Enzyme Immunoassay for the Quantitative Determination of purified M13 Bacteriophage Particles |
) AAV5 Titration ELISA Kit (Fast) |
| AAV-A005H | ACROBIOSYSTEMS | 96tests | EUR 719.4 |
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Description: AAV5 Titration ELISA Kit(Fast) is based on ELISA sandwich method and designed to detect and quantitative determine of AAV5 capsid titration in AAV gene therapy product preparation processing. It contains AAV5 empty capsids and a pair of antibodies against AAV5. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of AAV5 capsid titration. |
M13 Phage Titration ELISA Kit |
| MBS539756-5x96Tests | MyBiosource | 5x96Tests | EUR 6375 |
M13 Phage Titration ELISA Kit |
| MBS539756-96Tests | MyBiosource | 96Tests | EUR 1375 |
 Retrovirus qPCR Quantification Kit |
| K1472-100 | Biovision | 100 Rxns | EUR 381.6 |
) Rat Serum IgG detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80155-G | Alpha Diagnostics | 1 kit | EUR 781.2 |
) Rat Serum IgM detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80155-M | Alpha Diagnostics | 1 kit | EUR 781.2 |
 HiPer® Phage Titration Teaching Kit |
| HTM003E-10PR | EWC Diagnostics | 1 unit | EUR 84.26 |
Description: HiPer® Phage Titration Teaching Kit |
) Mouse Serum IgM detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80150-M | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Lentivirus Titration XpressCard |
| ATG-LT-01-10 | Antagene | 10 tests | EUR 195 |
Lentivirus Titration XpressCard |
| ATG-LT-01-20 | Antagene | 20 tests | EUR 335 |
Lentivirus Titration XpressCard |
| ATG-LT-01-50 | Antagene | 50 tests | EUR 715 |
Lentivirus Titration XpressCard |
| ATG-LT-01-6 | Antagene | 6 tests | EUR 136 |
) Mouse Serum IgG3 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80150-G3 | Alpha Diagnostics | 1 kit | EUR 781.2 |
 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)) Mouse Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80150-A | Alpha Diagnostics | 1 kit | EUR 781.2 |
 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)) Mouse Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80150-E | Alpha Diagnostics | 1 kit | EUR 781.2 |
 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)) Rat Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80155-A | Alpha Diagnostics | 1 kit | EUR 781.2 |
 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)) Rat Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80155-E | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Instant Lentivirus Titration Card |
| G614 | ABM | 10 Tests | EUR 235 |
Instant Lentivirus Titration Card |
| G615 | ABM | 20 Tests | EUR 405 |
 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)) Mouse Primary Antibody (IgG1) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80150-G1 | Alpha Diagnostics | 1 kit | EUR 781.2 |
 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)) Mouse Primary Antibody (IgG2a) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80150-G2a | Alpha Diagnostics | 1 kit | EUR 781.2 |
 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)) Mouse Primary Antibody (IgG2b) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80150-G2b | Alpha Diagnostics | 1 kit | EUR 781.2 |
 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)) Mouse Primary Antibody (A+G+M) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80150 | Alpha Diagnostics | 1 Kit | EUR 781.2 |
 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)) Rat Primary Antibody (A+G+M) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80155 | Alpha Diagnostics | 1 Kit | EUR 781.2 |
 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)) Rabbit Primary Antibody (A+G+M) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80160 | Alpha Diagnostics | 1 Kit | EUR 781.2 |
 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)) Mouse Primary Antibody (IgG1-3) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80150-G | Alpha Diagnostics | 1 kit | EUR 781.2 |
 baculoQUANT All-in-one virus extraction & titration kit |
| GWB-100602 | GenWay Biotech | 100 reactions | Ask for price |
 Antibody detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)) Ferret Primary Antibody (IgA) Antibody detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80188-A | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Antibody detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)) Ferret Primary Antibody (IgG) Antibody detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80188-G | Alpha Diagnostics | 1 kit | EUR 781.2 |
 Antibody detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)) Ferret Primary Antibody (IgM) Antibody detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
| 80188-M | Alpha Diagnostics | 1 kit | EUR 781.2 |
, 99.5%) Pyridine (For Karl Fischer Titration), 99.5% |
| 75453 | Sisco Laboratories | 500 ml | EUR 7.18 |
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Description: Part A |
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