Despite the observed serious effects of microgravity on mammalian cells, many astronauts have completed long-term stays in space without experiencing serious health problems. This raises questions about cell capacity of adaptation to a new gravitational environment. The International Space Station (ISS) experience Triple Lum A, carried out in the BioLab laboratory of the Iss Columbus module, allowed for the first time the direct measurement of a cellular function in real time and on orbit. We measured the oxidizing burst reaction in mammal macrophages (Rat NR8383 alveolar macrophages) exposed to a centrifuge system of 0 g and 1 g of internal commands and increase the stage and decrease of gravitational force.
In four independent experiments. Surprisingly, we found that these macrophages are adapted to microgravity ultra-fast in seconds, after an immediate inhibitor effect on the oxidizing burst reaction. For the first time, we have provided direct evidence of cell sensitivity to gravity, thanks to real-time measurements on orbits and using an experimental system, in which all factors except the severity were constant. The surprisingly ultra-fast adaptation to microgravity indicates that mammal macrophages are equipped with a very effective adaptation potential to a weak gravity environment. This opens up new avenues for exploring the adaptation of mammalian cells to gravitational changes.
Knowledge of analyte stability is crucial for interpreting biological analysis with confidence. However, centrifugation before transport takes time, and the transfer of plasma or serum of a primary tube to a secondary tube increases the risk of preanalytical errors. For stable analytes in total blood for 24 hours or more, it seems that there is no advantage to centrifuge before transport.
Cytocentrifuge Veterinary cytology preparation: a fast, simple and affordable manual method for concentrating low cellular fluids.
The smears prepared by cytocentrifugation, the so-called cytospins, are widely used in human and veterinary cytology. However, the high cost has hindered the availability of commercial cytospinal centrifuges in some veterinary clinics and laboratories. Nevertheless, cytospines are important for assessing fluids with very low cellularity such as cerebrospinal fluid (CSF) or bronchoalvolar washing fluid (BALF). Twenty-two fluid samples (including CSF and BALF) were collected from dogs and cats. These have been treated in a conventional cytocentrifuge and in an internal manual centrifuge (spinner with salad). The cytospines obtained by the 2 methods were compared by marking the cellularity, the number of cells per field, the hemodilution, the preservation of the cells and the proportion of broken cells.
In addition, the number of cells and the size have been compared by morphometry. The differences between the automated and manual method have been statistically evaluated. Manual cytocentrifuge has produced cytospines with similar cell efficiency as an automated cytocentrifuge. Considering low cost and low portability, this new method should be particularly useful for cytological diagnosis in small clinics, developing countries and field studies.

Prevalence of pre-analytical errors in clinical chemistry diagnostic laboratories in the city of Sulaimani of Iraqi Kurdistan.
Laboratory tests are roughly divided into three phases: a pre-analytical phase, a phase of analysis and a post-analytical phase. Most analytical errors have been assigned to the analytical phase. However, recent studies have shown that up to 70% of analytical errors reflect the pre-analytical phase. The pre-analytical phase includes all processes from the moment when a laboratory application is made by a doctor until the specimen is analyzed in the laboratory. As a general rule, the pre-analytical phase includes the preparation of the patient, the transport of samples, the collection and storage of the samples. In this study, we report the first complete assessment of the frequency and types of pre-analytical errors during the Sulaimani diagnostic laboratories of Iraqi Kurdistan.
Over 2 months, 5,500 venous blood samples were observed in 10 public diagnostic laboratories in the city of Sulaimani. The percentages of rejected samples and types of inadequacy of the sample were evaluated. The percentage of each of the following pre-analytic errors has been recorded: delay in the transport of samples, coagulated samples, exhaled reagents, hemolysis samples, samples not on the ice, identification of incorrect samples, insufficient sample, tube broken in the centrifuge, query procedure errors, Mix-UPS sample, communication conflicts, misinterpreted controls, labiamic samples, contaminated samples and missed doctor’s application orders. The difference between the relative frequencies of errors observed in the hospitals considered has been tested using a proportional Z test. In particular, the survey was intended to determine whether analytical errors were recorded and examine the types of platforms used in selected diagnostic laboratories.
Single-Channel Reagent Reservoir (boat) |
RB333 |
Bio Basic |
1 UNIT |
EUR 61.82 |
|
Single channel mini-pipette (130 mm) 10 ul, autoclavable |
SCMP-10 |
Alpha Diagnostics |
1 |
EUR 110.4 |
Biotin-XX Tyramide Reagent |
A8012-10 |
ApexBio |
10 mg |
EUR 199.2 |
Description: Biotin-LC-LC-tyramide is used for tyramide signal amplification (TSA) which is a powerful, patented technology that significantly enhances both chromogenic and fluorescent signals. TSA is easily integrated into standard nonradioactive in situ hybridization (ISH) or IHC protocols |
Biolipidure-1002-Reagent |
Biolipidure-1002-10 |
Cusabio |
10mL |
EUR 235.2 |
|
Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
Biolipidure-103-Reagent |
Biolipidure-103-10 |
Biolipidure |
10mL |
EUR 235.2 |
|
Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
Biolipidure-1201-Reagent |
Biolipidure-1201-10 |
Biolipidure |
10mL |
EUR 235.2 |
|
Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
Biolipidure-1301-Reagent |
Biolipidure-1301-10 |
Biolipidure |
10mL |
EUR 235.2 |
|
Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
Biolipidure-203-Reagent |
Biolipidure-203-10 |
Biolipidure |
10mL |
EUR 235.2 |
|
Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
Biolipidure-206-Reagent |
Biolipidure-206-10 |
Biolipidure |
10mL |
EUR 235.2 |
|
Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
Biolipidure-405-Reagent |
Biolipidure-405-10 |
Biolipidure |
10mL |
EUR 235.2 |
|
Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
Biolipidure-502-Reagent |
Biolipidure-502-10 |
Biolipidure |
10mL |
EUR 235.2 |
|
Description: The Biolipidure-502 Reagent is a synthetic cationic polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
Biolipidure-702-Reagent |
Biolipidure-702-10 |
Biolipidure |
10mL |
EUR 235.2 |
|
Description: The Biolipidure-702 Reagent is a synthetic amphoteric polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
Biolipidure-802-Reagent |
Biolipidure-802-10 |
Biolipidure |
10mL |
EUR 235.2 |
|
Description: The Biolipidure-802 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-802 generally enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, Rapid-test, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
2 channel flow cell with temp sensor |
FC3020T |
Consort |
ea |
EUR 321.6 |
Girard?s Reagent P-d5 |
B8291-10 |
ApexBio |
10 mg |
EUR 226.8 |
Storage Rack For LS750 |
TW-R036-9C34 |
MiTeGen |
1 UNIT |
EUR 163 |
|
Description: Storage Rack For LS750 |
Storage Rack For LS3000 |
TW-RS30-9C50 |
MiTeGen |
1 UNIT |
EUR 218 |
|
Description: Storage Rack For LS3000 |
Storage Rack For LS6000 |
TW-RS60-9C50 |
MiTeGen |
1 UNIT |
EUR 308 |
|
Description: Storage Rack For LS6000 |
HemogloBind™ Blood Card Reagent Kit |
H0145BC-10 |
Biotech Support Group |
10 preps |
EUR 540 |
Description: Hemoglobin Removal Kit |
HemoVoid™ - Blood Card Reagent Kit |
HVBC-10 |
Biotech Support Group |
10 preps |
EUR 662.4 |
Description: Hemoglobin Removal Kit |
10-100ul Filter Tip, Natural, Sterile, 96/Rack x 10 |
FT224-RNSB |
Bio Basic |
1PK, 960UNIT |
EUR 118.49 |
|
Reagent Reservoirs 12 Channel Basin, Sterile, Individually wrapped |
LC2003-020 |
GenDepot |
20/pk |
EUR 343.2 |
ExoPure? Reagent (Overall Exosome Isolation, biological fluids) |
M1001-10 |
Biovision |
|
EUR 796.8 |
Bax channel blocker |
A4459-10 |
ApexBio |
10 mg |
EUR 408 |
Description: IC50: 0.52 ?M in Bax assayBax channel blocker is an inhibitor of Bax-mediated mitochondrial cytochrome c release. |
Corning DWorks 1-200uL Pipet Tips Graduated Hinged Rack Sterile 96tip/Rack 10 rack/Pack 4pack/Case |
4121 |
Scientific Laboratory Supplies |
PK3840 |
EUR 296.4 |
10-100UL SINGLE CHANNEL PIPETTOR |
AP-100 |
CORNING |
1/pk |
EUR 218.4 |
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
1730 10 SNAP-SEAL 10 OZ |
1730-10 |
CORNING |
100/pk |
EUR 96 |
Description: Disposable Plastic; Plastic Containers |
99445-10 DCT 10 X 75MM |
99445-10 |
CORNING |
250/pk |
EUR 75.6 |
Description: Disposable Culture Tubes; DCT's, CGW |
AXYGEN® AXYPET® PRO 0.5-10 ΜL SINGLE-CHANNEL PIPETTOR, AUTOCLAVABLE |
AP-10-P |
CORNING |
1/pk |
EUR 242.4 |
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
GSA 10 |
B5767-10 |
ApexBio |
10 mg |
EUR 274.8 |
SC-10 |
B6299-10 |
ApexBio |
10 mg |
EUR 255.6 |
MRT 10 |
B7685-10 |
ApexBio |
10 mg |
EUR 285.6 |
AXYGEN® GEL TRAY 10 X 10 CM FOR USE WITH 10 CM GEL BOX, UV TRANSPARENT |
HGB10-10-GT |
CORNING |
1/pk |
EUR 88.8 |
Description: Lab Equipment; Axygen Branded EQ |
300ul Natural Tips, Sterile, 96/Rack x 10 |
BT330-RNSB |
Bio Basic |
1PK, 960UNIT |
EUR 116.93 |
|
10-100ul Filter Tip, Natural, Sterile, 96/Rack |
FT224-RNS |
Bio Basic |
1PK, 96UNIT |
EUR 64.7 |
|
AXYGEN® AXYPET® PRO 0.5-10 ΜL, 12 CHANNEL PIPETTOR, AUTOCLAVABLE |
AP-12-10-P |
CORNING |
1/pk |
EUR 580.8 |
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
AXYGEN® AXYPET® PRO 0.5-10 ΜL, 8 CHANNEL PIPETTOR, AUTOCLAVABLE |
AP-8-10-P |
CORNING |
1/pk |
EUR 546 |
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
Keratin 10 antibody |
10-2541 |
Fitzgerald |
250 ug |
EUR 590.4 |
Description: Mouse monoclonal Keratin 10 antibody |
AI-10-49 |
A8694-10 |
ApexBio |
10 mg |
EUR 254.4 |
Description: AI-10-49 is a selective inhibitor of CBF? -SMMHC and RUNX1 interaction with a FRET IC50 value of 260nM. AI-10-49 restores RUNX1 transcriptional activity, displays favorable pharmacokinetics, and delays leukemia progression in mice. |
MFZ 10-7 |
B5595-10 |
ApexBio |
10 mg |
EUR 420 |
TC ASK 10 |
B5728-10 |
ApexBio |
10 mg |
EUR 525.6 |
PSB 10 hydrochloride |
B6923-10 |
ApexBio |
10 mg |
EUR 439.2 |
10-DEBC hydrochloride |
B7105-10 |
ApexBio |
10 mg |
EUR 265.2 |
Description: 10-DEBC hydrochloride is a selective inhibitor of Akt (or termed PKB) [1], with an IC50 value of approximate 48 ?M [2].Akt is a type of serine/threonine kinase. It phosphorylates and inactivates components in the apoptotic machinery, including Caspase 9 and BAD. |
UNIVERSAL CAROUSEL RACK FOR SIX PIPETTES |
4078 |
CORNING |
1/pk |
EUR 158.4 |
Description: Corning and Axygen Liquid Handling Equipment; Corning Lambda Plus Single Channel Pipettors |
UNIVERSAL LINEAR RACK FOR SIX PIPETTES |
4079 |
CORNING |
1/pk |
EUR 84 |
Description: Corning and Axygen Liquid Handling Equipment; Corning Lambda Plus Single Channel Pipettors |
Rack for 30x15 & 20x50ml (17x20x6cm), Orange |
R1070 |
MTC Bio |
5/pack |
EUR 104.26 |
Description: Rack for 30x15 & 20x50ml (17x20x6cm) Orange |
Over 2 months, 5,500 venous blood samples were observed in 10 public diagnostic laboratories in the city of Sulaimani. The percentages of rejected samples and types of inadequacy of the sample were evaluated. The percentage of each of the following pre-analytic errors has been recorded: delay in the transport of samples, coagulated samples, exhaled reagents, hemolysis samples, samples not on the ice, identification of incorrect samples, insufficient sample, tube broken in the centrifuge, query procedure errors, Mix-UPS sample, communication conflicts, misinterpreted controls, labiamic samples, contaminated samples and missed doctor’s application orders. The difference between the relative frequencies of errors observed in the hospitals considered has been tested using a proportional Z test. In particular, the survey was intended to determine whether analytical errors were recorded and examine the types of platforms used in selected diagnostic laboratories.