An efficient rearing system rapidly producing large quantities of poultry red mites, Dermanyssus gallinae (Acari: Dermanyssidae), under laboratory conditions.

The red mite of poultry, Dermanysus gallinae, is one of the most economically deleterious ectoparasites that affect laying chickens of eggs in many parts of the world. New approaches to D. gallinae control often require the maintenance of D. gallinae colonies under laboratory conditions. In this study, we present an effective breeding system for D. gallinae, consisting of a metal cage, a plastic storage box and a tray filled with water. The chicks were raised in the cage as host animals. A new trap has been developed to monitor the dynamic changes of mite populations, consisting of a plastic centrifugal tube and a disposable breathing mask with folds. The mites parameters were analyzed, including mites and eggs, survival rates and food, ovipos, burst and proportion of D. gallinae at different stages of life. The results show that the livestock system had a 53.5% increase in the number of mites over a six-week period after the introduction of mites.

The survival rates of mites were greater than 94% and the average feed rates ranged from 22.57% to 37.30%. The average number of eggs per woman ranged from 3.42 to 3.50, with the bursting of eggs greater than 97%. Nymphs were the majority of the population, ranging from 71.46% to 81.37%, while the larvae population was minor and ranging from 7.54% to 13.04%. The mask trap used in this study was an efficient and practical device for sheltering D. gallinae and monitor the dynamic changes of the mascian population. The livestock system has proved very effective in maintaining and reproducing colonies from D. gallinae, with great potential for assessing the efficacy of vaccines or compounds against D. gallinae under laboratory conditions. This would be a useful tool for narrow observations in studies on the biology, the acology and physiology of red mites of poultry.

 

Automated end-to-end blood test at the point of care: Integration of robotic phlebotomy with downstream sample processing.

The diagnostic blood test is the most commonly carried out clinical procedure in the world and influences the majority of medical decisions taken in hospitals and laboratory. However, the manual success rates of the blood draw depend on the competence of the clinicians and the physiology of patients, and the results are generated almost exclusively in centralized laboratories from large samples using high-intensity analytical techniques. labor. This article presents a medical device that allows end-to-end blood tests by performing blood draws and providing diagnostic results in a fully automated way at the point of care. The Torque system A VEINUNCURE ROBOTAGE by image, developed to meet the challenges of routine venous access, with a centrifuge-based blood analyzer to obtain quantitative measurements of hematology.

We first demonstrate a dosage of white blood cells on the analyzer, using an excessive fluorescent microbail fluid, where the surface of the packaged ball layer is correlated with the pearl concentration. Then we perform experiments to evaluate the pumping efficiency of the sample manipulation module. Finally, studies are carried out on the integrated device – of the blood draw at the analysis – using ghosts of blood vessels to evaluate the accuracy and repeatability of the resulting white blood cell test. Trypanosomiasis is an important protozic disease with a diverse range of sensitive host, including man. In the current study, the molecular characterization of the spreading species has been made through a reaction of the Pan-trypanosome chain of the polymerase (PCR) followed by a polymorphism of restriction fragment length (RFLP). In total, three hundred equines, including horses, donkeys and mules, have been randomly selected and equine blood samples have been subjected to trypanosomal screening through the micro-tomatology centrifuge technique, the PCR conventional, semi-nestled PCR and RFLP.

An efficient rearing system rapidly producing large quantities of poultry red mites, Dermanyssus gallinae (Acari: Dermanyssidae), under laboratory conditions.

Tandem B and Tandem B and T High Purity isolate for a QRT-PCR analysis adapted to fundamental laboratories.

Malaria is still a major health problem in sub-Saharan Africa and Southeast Asia, but research on malaria in low-income countries can be a challenge due to lack of laboratory equipment. In addition, severe malaria mainly affects very young children, which limits the amount of blood available for research purposes. Thus, there is a need for protocols that produce a maximum of information from a minimum amount of blood, which are suitable in essentially equipped laboratories.

A protocol for cell insulation of cell B and th) directly from the total blood and a method of preserving independent independent samples of the freezer compatible with the hot and humid climate of the malaria regions has been established and validated. The protocol thus rotates the need for high-tech centrifuges and a large diet for the isolation of mononuclear cells in the peripheral blood. Purity and performance are excellent. According to the level of expression of genes of interest, between 2 and 5 ml of blood are adequate for reliable QRT-PCR results of the B and TH cells of healthy pediatric donors as well as patients with pediatric malaria.

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This protocol for high high-purity B high-yield cell isolation B and sample storage for subsequent QRT-PCR analysis from a minimum amount of blood is invented with basic equipment and independently of the continuous supply. Thus, many scientists are likely to research malaria in rural institutes or hospitals, and therefore in countries where malaria is the most widespread. Although the rejection of viral load samples has been improved from 1.8 to 0% until February / 2018, the problem was rewarded and pursued at the end of May (1.3%) and June (0.3 %) 2018. In addition, the rejection of CD4 samples (4.3%) was outside the target established in May and the rejection of the diagnostic samples of the infants exposed (EID) has become increased since March 2018. Therefore, Appropriate corrective and preventive actions and close monitoring could reduce the problem of the specimen reference network.

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